Antinuclear Cytoplasmic Antibody

Pathophysiology

ANCA binds explicitly to two proteins in the cytoplasm of neutrophils in ANCA associated vasculitis, named proteinase-3 (PR3) and myeloperoxidase (MPO).[3] These patients have autoantibodies against PR3 (PR3-ANCA) or MPO (MPO-ANCA) at a time.[3] PR3-ANCA is more found explicitly in granulomatosis with polyangiitis (GPA or Wagner’s Granulomatosis) than others.[4] MPO-ANCA can be found in microscopic polyangiitis (MPA), eosinophilic granulomatosis with polyangiitis (EGPA or Churg Strauss Syndrome) more frequently than in GPA.[4]

Small vessel damage caused by ANCA occurs when these autoantibodies bind to granules in neutrophils, the mechanism of pathogenesis is given below[5][6]:

• Neutrophils and monocytes activation by ANCA
• Subsequent activation of the alternative complement pathway
• Neutrophils migration through blood vessel walls and the release of inflammatory mediators
• Release of chemoattractant and recruitment of more inflammatory mediators
• Inflammation, apoptosis, necrosis, and damage to small blood vessel walls

Recently it has been discovered that few medications can cause drug-induced anti-neutrophil cytoplasmic antibodies (ANCA)-associated vasculitis (AAV). The drug-induced AAV is seen with propylthiouracil, hydralazine, and D-penicillamine.[7][8][9] Its pathogenesis is multifactorial. Drug-induced AAV is reversible after medication discontinuation, so it has a good prognosis.[7]

There are two following subtypes/patterns of ANCA[2][10]:

1. c-ANCA (cytoplasmic pattern on IIF), which targets PR3 (proteinase 3) in neutrophilic cytoplasm
2. p-ANCA (perinuclear pattern on IIF), which targets MPO (myeloperoxidase) in neutrophilic cytoplasm

ANCA lab testing shows which type of antibody is present, which then narrows down the differential diagnosis.

Specimen Requirements and Procedure

A blood sample is taken from a peripheral venous draw. A healthcare professional puts a rubber band around the arm and asks the patient to keep pumping fist for some time. Healthcare professional palpates the vein for confirmation and then applies alcohol swabs to clean the area. After it has dried up, the phlebotomist introduces a needle into the vein and collects a blood sample in a test tube or a vial. After collecting the sample, the band is removed, the pressure is applied over the puncture site, hemostasis is secured, and a bandage is applied. Complications of the procedure include mild tenderness, bruising, oozing of blood or infection at the puncture site.

Testing Procedures

After taking a blood sample, it undergoes analysis by two techniques[3]:

1. Indirect immunofluorescence (IIF)
2. Enzyme-linked immunosorbent assay (ELISA)

The indirect immunofluorescence (IIF) identifies ANCA by its staining patterns inside the cytoplasm of neutrophils[10]. In this technique, neutrophils are fixed via ethanol onto a slide, serum of blood sample is mixed on the slide with the neutrophils and ANCA, if present, in the sample attach to neutrophils on the slide. The slide is then treated with fluorochrome-stained antibodies which react with ANCA on the slide; this forms a fluorescence pattern that is visible via a microscope. As mentioned earlier, if ANCA staining is throughout the cytoplasm, it is called a c-ANCA pattern, mostly with PR3-ANCA. If staining is only around the nucleus, it is called p-ANCA (perinuclear pattern), which usually occurs with MPO-ANCA.[10] On the other hand, ELISA measures the level of MPO-ANCA or PR3-ANCA patterns in a blood sample.

Testing takes place in two steps. First indirect immunofluorescence (IIF) is done, which detects the presence or absence of these autoantibodies, and if the test comes positive, then it is followed by ELISA.[11] The International Consensus Statement on Testing and Reporting ANCA recommends using both techniques to detect ANCA in suspected patients.[12]

Interfering Factors

ANCA testing helps diagnose and monitor small vessel vasculitis, but it has some limitations too. There is a variant of ANCA called atypical ANCA, which is associated with drug-induced ANCA-associated vasculitis (AAV), inflammatory bowel disease, and rheumatoid arthritis.[13][14] ANCA positive rate is also higher in type 1 diabetes mellitus than in healthy individuals.[15]

Results, Reporting, and Critical Findings

Negative IIF result, i.e., ANCA is not detected: Normal.

Positive IIF result, i.e., ANCA is detected: Abnormal (In this case, the fluorescence pattern is also given to show whether c-ANCA or p-ANCA is present).

MPO Antibody (p-ANCA) Levels

• >5.0 IU/ml = Positive
• 3.5-5 IU/ml = Equivocal
• <3.5 IU/ml = Negative

PR3 Antibody (c-ANCA) Levels

• >3.0 IU/ml = Positive
• 2-3.0 IU/ml = Equivocal
• <2.0 IU/ml = Negative

Interpretation

Positive results for c-ANCA are mostly consistent with granulomatosis with polyangiitis (GPA or Wegener granulomatosis). Whereas positive results with p-ANCA mostly occur in microscopic polyangiitis (MPA) and eosinophilic granulomatosis with polyangiitis (EGPA or Churg-Strauss syndrome). Monitoring the titers of ANCAs are useful in monitoring the clinical course of diseases.

Clinical Significance

Positive ANCA lab results with clinical suspicion aids in diagnosing some small vessel vasculitis, including pauci-immune small vessel vasculitides granulomatosis with polyangiitis (GPA or Wegener granulomatosis), microscopic polyangiitis (MPA), eosinophilic granulomatosis with polyangiitis (EGPA or Churg-Strauss syndrome) and anti-glomerular basement disease.[1][2] The reappearance of ANCA after the treatment of vasculitis can indicate a relapsing disease.[16][17]