Embryology, Gastrointestinal

Development

During the first three weeks of fetal development, implantation of the blastocyst occurs. This occurs around the same time as secretion of hCG, which is the hormone detected for pregnancy tests. During the second week, the bilaminar disc forms, which includes the epiblast and hypoblast. During the third week of development, gastrulation occurs; the epiblast cells invaginate, replace the hypoblast, and proliferate into the middle layer, while the primitive streak forms the ectoderm. This process of gastrulation leads to the development of the three germ layers: ectoderm, mesoderm, endoderm. These layers form different parts of each system, as discussed above.

During week four, the heart begins to beat and can be detected by ultrasound by week six. Also during week four, limb buds begin to form. By week eight fetal movements begin and by week ten, the genitalia forms.

The changes that occur during embryology take place simultaneously. Organogenesis (development of the organs) occurs from weeks three to eight. Avoiding teratogens especially during these weeks is crucial. The following are the embryological changes that occur specifically with the gastrointestinal tract.

• Week 3: the digestive tube starts differentiating. Gastrulation occurs. Initially, the prime gut tube forms as a hollow cylinder of endodermal cells surrounded by mesoderm. The endoderm sheet elongates and folds ventrally at the anterior and posterior ends, meeting near the yolk sac to form a closed tube.[1]

• Week 4: resorption of the buccopharyngeal membrane occurs, which closes the cranial end of the digestive tube.[2]

• Week 6-10: midgut herniates throughout the umbilical ring, where it develops almost entirely outside the peritoneal cavity, then rotates back around in week ten. 

• Week 7: obliteration of the omphalomesenteric duct (vitelline duct), which connects the midgut lumen to the yolk sac

• Week 9: Opening of the distal cloacal membrane.[2] Villus formation begins[3]

• Week 11: distinctive longitudinal and circular muscle layers are present through the intestines

• Week 12: crypt development begins.

• Week 14: muscularis mucosae develops

• Week 24: fetal intestinal absorption function develops

• Week 32: fetal intestinal absorption reaches adult level.[4]

Gastrointestinal development also includes the development of the enteric nervous system. The enteric nervous system (ENS) includes two ganglion neuron networks: the location of the myenteric (Auerbach) plexus is between the inner circular and the outer longitudinal muscle layers, the submucosal (Meissner) plexus is situated adjacent to the mucosal layer. These cells derive from the neural crest cells. The enteric nervous system is developed through many complex mechanisms, such as axon guidance and synaptogenesis, leading to the proliferation, migration, and differentiation of ENS precursors. These plexuses work with smooth muscle cells and mucosal villi to control the absorption and secretion of the GI system. The smooth muscle cells are derived from mesoderm and differentiate in a rostral to caudal wave.[3]

Cellular

Different epithelial cell types are present by 12 weeks and are resembling adult cells of the intestine by 22 weeks.

Enteric nervous system development, as well as that of other cells in the gut, is regulated by different factors, including the sonic hedgehog and Indian hedgehog, bone morphogenetic protein, Wnt signaling, and platelet-derived growth factor. The hedgehog signaling is vital for early left and right patterning, along with villi formation. The bone morphogenetic protein is essential for dorsal-ventral patterning. Wnt signaling is involved in anterior-posterior signaling, villi formation, and epithelial stem cell proliferation in the intestines. PDGF plays a significant role in smooth muscle differentiation.

These signaling pathways are essential for the cell systems to develop and integrate for GI function.[3]

Testing

• Fetal kidneys produce amniotic fluid, and it is swallowed through the mouth by the fetus. A defect in the kidneys can lead to a low quantity of amniotic fluid (oligohydramnios), and defective swallowing of the amniotic fluid can lead to excess amniotic fluid (polyhydramnios)
• The diagnosis of a GI obstruction is possible in the late second to early third trimester.
• Amniotic fluid can be assessed to help check fetal health. There are many ways to assess the quantity of amniotic fluid. An ultrasound can provide accurate data about the amount of amniotic fluid. 
  • Clinical palpation to measure the single deepest vertical pocket
  • Amniotic fluid index (AFI) using the four-quadrant technique; this is the most popular and reliable method.
    • AFI <5 cm is oligohydramnios
    • AFI >25 cm is polyhydramnios
    • AFI between 8-25 is considered normal.[5]
• Fetal MRI can be used to complement the findings of an ultrasound.[6]

Pathophysiology

There can be many defects that take place during embryogenesis that affect the GI tract.

• Atresia is the absence or abnormal growing of an opening or a passage.

1. Ventral wall defects due to a failure of closure
   1. Gastroschisis: contents protrude through abdominal folds and not covered by amnion or peritoneum.
   2. Omphalocele: abdominal contents fail to return to the abdomen and are sealed by peritoneum
2. GI atresia is a failure of a portion of the GI tract to develop. The bowel will be distended at the level at the level of obstruction, while distal segments will be less distended.[2]
   1. Esophageal atresia has an association with tracheoesophageal fistula which allows air to enter the stomach
   2. Duodenal atresia occurs due to failed recanalization. It has an association with a “double bubble” sign as well as with Down syndrome.
   3. Jejunal and ileal atresia occurs when there is disruption of the mesenteric vessels leading to necrosis
3. Hirschsprung’s disease
   1. Occurs with altered peristalsis
   2. Lack of neural crest-derived- ganglion neurons in a part of the colon due to abnormal migration, differentiation, or proliferation of neural crest cells during embryogenesis.[7]
4. Hypertrophic pyloric stenosis
   1. Acquired narrowing of the pylorus
   2. The presentation includes projectile, nonbilious vomiting with a palpable olive mass in the abdomen
   3. May include a peristaltic wave after being fed.[8]
5. Meconium ileus
   1. Is associated with cystic fibrosis
   2. Defined as the lack of passage of stool at birth due to obstruction of meconium.[9]
6. Malrotation of the gut
   1. Improper positioning of the bowel leading to the formation of fibrous bands
7. Volvulus
   1. Twisting of bowel around its mesentery
8. Vitelline fistula
   1. Vitelline duct remains patent.
   2. Clinical presentation with meconium discharge through the umbilicus.[10][6]

Clinical Significance

Many different pathophysiological processes can occur during embryogenesis of the GI tract. Many of these can are correctable by surgery and do not cause fetal demise in utero. A few of these embryological defects have associations with other diseases, such as cystic fibrosis and Down syndrome.  Therefore, babies with meconium ileus and duodenal atresia require close monitoring and further evaluation for cystic fibrosis and Down syndrome, respectively.

It is vital during pregnancy to get routine ultrasounds to assess for polyhydramnios to suspect any GI anomalies. If a GI anomaly is suspected, it is important to monitor closely for any future surgeries needed for the baby.[10]